Ceramide assay kit
WebThis Human Phosphatidylcholine:ceramide cholinephosphotransferase 1 (SGMS1) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. WebCeramides were eluted, separated, and detected using a triple quadruple mass spectrometer equipped with positive ion electrospray ionization (ESI) and selected …
Ceramide assay kit
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WebMar 2, 2024 · Ceramides (Cer) belong to the group of ceramide lipids within the sphingolipids. Their structure consists of a long-chain base which is linked to a fatty acid … WebNov 1, 2001 · After the addition of 1 ml of 1 mol/l NaCl and 1 ml of CHCl 3 and further mixing, phases were separated by centrifugation at 5,000g for 2 min. Lipid extracts in the lower phase were assayed for ceramide content using a radiometric assay kit according to the manufacturer’s instructions, as DAG kinase can phosphorylate both DAG and …
WebTo achieve ER labeling, this kit employs a primary antibody directed against an ER-associated protein, protein disulfide isomerase (PDI), and an Alexa Fluor 488 dye–labeled secondary antibody. The Alexa Fluor 488 dye exhibits bright green fluorescence that is compatible with filters and instrument settings appropriate for fluorescein. WebELISA Kits; Assay Kits; Biomolecules; Inhibitors; Recombinant Proteins; Chemicals and Reagents; Enzymes; Cells and Microorganisms; Blood and Tissue Products; Nucleic …
WebThe assay will exclusively recognize both natural and recombinant human Alpha-Galactosidase A/GLA. Target information This gene encodes a homodimeric … WebMar 30, 2024 · Ceramides involve a family of lipids consisting of sphingosine covalently linked to a fatty acid that regulates pleiotropic cellular events, including cell differentiation, proliferation, and...
WebPlasma ceramides are predictors of adverse cardiovascular events resulting from unstable atherosclerotic plaque. Ceramides are complex lipids that play a central role in cell …
WebThe CerS Assay Kit provides an easy to use assay for measuring CerS activity in biological samples. This Kit offers the advantage of a short assay time requiring only small … toy hackerWebAssay Range: 0.0625 µM – 2 µM Sample Volume: For duplicate data points 25 µL of each sample is required. Sample Type: This kit detects S1P from human or animal in serum, plasma, tissue homogenate, and cell lysate, … toy hacking robotWebMar 21, 2003 · After the subsequent addition of 0.25 ml of 1 m NaCl and 0.25 ml of CHCl 3, phases were separated by centrifugation at 3000 rpm (2000 × g) for 5 min. Ceramide content in the extract was determined using a radiometric diacylglycerol assay kit (Amersham Biosciences) according to the manufacturer's instructions. toy hackathonWebCell Navigator® TMR Ceramide Golgi Staining Kit provides an optimized assay method for examining the morphology of the Golgi apparatus with a fluorescence microscope. In … toy habits youtubeWebApr 13, 2024 · The literature data indicate that the cellular levels of sphingosine-1-phosphate (S1P) and ceramides (Cer) play a significant role in the pathogenesis of CRC, and the S1P/Cer ratio (described as sphingolipid rheostat) decides whether the cells will be directed towards proliferation or cellular senescence and apoptosis [ 6, 7 ]. toy guns with holster and beltWebOct 22, 2024 · Ceramide labeling in patient-derived fibroblasts showed an altered distribution of ceramide indicated by the increased puncta compared to control … toy hackingWebOct 15, 1999 · A fluorescence-based high-performance liquid chromatographic assay to determine acid ceramidase activity Acid ceramidase (N-acylsphingosine amidohydrolase) is the lysosomal enzyme required to hydrolyze the N-acyl linkage between the fatty acid and sphingosine moieties in ceramide. toy hacks